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Molecular Diagnostics Technologist (MDT) — Requirements & Free Practice

US · License preparation

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Molecular Diagnostics Technologist (MDT)

AMT national certification for molecular diagnostics technologists.

Licensing authority: American Medical Technologists · Application information

Initial by examination
AMT examination for MDT
Eligibility
One of four AMT MDT education and experience routes must be met: Medical Laboratory Scientist Certification, Molecular Science Program, Laboratory Science Education, or Advanced Science Degree; all applicants must pass the MDT examination.

AMT examination for MDT

Provider
Pearson VUE
Format
Computer-based, four-option multiple-choice
Exam questions
Total questions: 200.
Passing score
Scaled score of 70 on a 0-100 scale
Examination outline
  • General Laboratory (18%)
    • Safety
    • Reagents and Supplies
    • Quality Assurance and Quality Control
    • HIPAA compliance
    • Safety Data Sheets (SDS)
    • Laboratory equipment and instrumentation
    • Policies and Procedures
    • Basic dilutions
  • General Molecular Diagnostics Theory (20%)
    • DNA/RNA
    • Proteins and protein expression
    • Endonucleases and exonucleases
    • Basic genetics
    • Infectious diseases
    • Oncology
    • Pharmacogenomics
  • Molecular Laboratory Procedures (10%)
    • Contamination and carryover
    • Molecular and biological contaminants
    • Workflow and physical separation
    • QC metrics
    • Specimen acceptability and preservation
    • Laboratory-developed test validation and reporting
    • Pipette technique
    • Proficiency testing
    • Regulatory requirements
    • DNA and RNA extraction procedures
  • Diagnostic Methods (52%)
    • Molecular diagnostic assay utility and limitations
    • Primer design
    • Primer performance
    • Reagent reconstitution
    • Controls and acceptability
    • Positive and negative cutoffs
    • Quantitative test calibrations
    • Positive and negative rate statistics
    • Standard curves and linearity
    • microRNA
    • Housekeeping genes
    • Mutation references
    • Polymerase chain reaction
    • Assays
    • Sequencing
    • Microarrays
    • Additional Techniques
    • DNA/RNA extraction
Examination sources